Layers - Salmonella Testing Requirements for Pullet Rearing Units

Litter Birds

Post Cleaning and prior to a new flock going into the house:

Please take:

1 gauze swab or sponge pad, from EACH of the following 6 areas PER HOUSE

  • Floors for litter houses (including cracked areas), or manure belts/droppings boards for cage
  • Walls (including cracked areas)
  • High beams/ledges and pipe-work
  • Fans & fan housing
  • In-house open feed hoppers/feeders
  • Drinkers

These may be tested in one or more composites.

Also take up to 25g of rodent faeces, from surfaces in house, or service area if none available in house.
If none available, producer is to sign a declaration and take gauze swab from areas around bait boxes.

If a previous flock has tested positive for Salmonella Enteritidis or Salmonella Typimurium. Please see us for an alternative testing kit.

Day Olds

Take a minimum of 10 Hatcher Tray Liners (minimum of A5 paper size) to be sampled in Hatchery and forward in sealed plastic bag with the chick delivery vehicle to site.
These may be tested in one or more composites.

PLUS – a maximum of 60 Dead on Arrivals from each hatchery.

14 weeks (Approx 2-3 weeks prior to transfer)

Please take a minimum of 2 pairs of boot swabs PER HOUSE AND 50g of dust or all readily available dust if less is available, collected from at least 10 locations in EACH HOUSE before dusting and sweeping.

Boot swabs to be taken from each flock (house or separate biosecure section of the house). The boot swabs must be moistened before sampling is carried out- clean tap water is suitable for this but some sampling kits will have pre-moistened boot swabs. Ensure there can be no contamination of swabs prior to use and that they cannot come into contact with disinfectant.

Boot swabs should represent the whole area to which the birds have access. This will include all the separate pens, littered and slattered areas (when they are safe to walk on) and involves taking about 100 paces to cover an area of approximately 50% of the house. Take the swabs before replenishing bedding.


All samples should be dispatched within 24 hours of collection. The BEIC Lion Code require all producers to ensure that the time between sampling and the lab starting to process the sample to be no later than 48 hours

For samples that fall outside of this time frame will not be able to have the laboratories UKAS accreditation mark on their salmonella results.

To avoid this please ensure samples are sent and received within 48 hours of sampling, it is therefore, not advisable to send samples on a Friday or Saturday, as they will not be tested until the following Monday, which will result in the 48 hour rule being breached.